推荐回答(1个)
目前自交不亲和系亲本的繁育主要靠人工蕾期授粉,但采用这种方法存在工作量大、效率低、繁殖成本高等问题,为了解决这些问题,国内外育种者在克服甘蓝自交不亲和系的不亲和性方面做了大量的研究。
国外学者有采用在花期提高CO2浓度若干小时的方法以提高结实(Nakanishi & Hinata,1975),但这要求一定的空间密闭性,实施起来有一定难度,且需找出适宜的CO2浓度和施用时间。
张恩慧等(1996)试验在自交不亲和系北大7220-2-5-2和黑叶小平头7221-1-3-1的花期喷5%食盐水加0.3%硼砂溶液,不仅可提高其亲和指数,而且还能够改善种子生活力。曹必好等(1998)在结球甘蓝自交不亲和系80-8-7的花期,喷5%和8%食盐水溶液均可克服其不亲和性,同时在盐水中加入0.3%硼砂,既可提高其亲和指数,又可增加种子的千粒重,并提高种子的生活力。徐文娟等(1999)在4个自交不亲和系:黑叶小平头7221-3-4-2、黑叶7321-4-2-2、黑叶78-3-13-8及79-103的花期,喷5%~6%的盐水加0.2%硼砂,能提高结球甘蓝自交不亲和系的结实指数。孟平红等(2003)以黔甘系列甘蓝的3个自交不亲和系A2-13-1(亲和指数0.11)、E4-2-f(亲和指数0.78)和G47-4(亲和指数0.12)为试材,在其花期喷洒5%的盐水加0.3%的硼砂水溶液,不仅可以克服甘蓝自交不亲和性,提高甘蓝自交不亲和系的结实指数,而且还能改善种子活力,提高种子的发芽率。秦甘80甘蓝的两个自交不亲和系亲本分别为B25-2-3-3-2和FT63-5-8-3-3,王妍妮等(2004)在花期以4%盐水+0.3%硼砂溶液喷B25-2-3-3-2系和以5%盐水+0.3%硼砂溶液处理FT63-5-8-3-3系,也显著提高了其亲本的亲和指数及种子千粒重、发芽势和发芽率。
另外,朱利泉等(1997)、华志明(1999)、吕俊等(2001)采用蛋白激酶抑制剂(槲皮素)和蛋白激酶激活剂(佛波酯)化学调控法分别对甘蓝自交不亲和系(00-62、00-A)与甘蓝自交亲和系(00-82)花蕾进行田间处理。结果表明,槲皮素处理后的自交不亲和系的自交亲和指数与对照相比有所提高,甚至转变为自交亲和系;相反,佛波酯处理后的自交亲和系的自交亲和指数与对照相比有所降低,甚至转变为自交不亲和系,差异显著性测验结果显示,处理与对照之间的结荚率和自交亲和指数均有显著差异。李成琼等(2005)对甘蓝自交不亲和系02-E1、02-A1、02-59在开花前两周的花蕾进行1500μmol/L槲皮素处理,能显著提高3个自交不亲和系的亲和指数;并采集开花前1d的花蕾切取柱头进行活性测定,结果表明,槲皮素处理后可显著抑制甘蓝自交不亲和系柱头中SRK活性,从而阻止了自交不亲和性的信号传导,达到了克服甘蓝自交不亲和性的目的。吴能表等(2004)通过槲皮素处理,研究了自交不亲和甘蓝自花授粉和异花授粉与蛋白激酶的关系,结果表明,槲皮素能明显抑制自交不亲和甘蓝自花授粉引起的蛋白激酶活性,导致蛋白质磷酸化明显受阻,进而促进自花诱导的花粉萌发和花粉管生长,同时使SOD、POD、CAT活性升高,Ca2+含量下降,Ca2+在胞内的分布明显改变,而槲皮素对异花授粉抑制作用不甚明显。因此,自交不亲和甘蓝阻止自花花粉萌发的机制可能与授粉后提高蛋白激酶活性和蛋白质磷酸化有关。这为甘蓝自交不亲和系的繁殖提供了新的思路和方法。
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