两者都可以用作疑问句的开头。区别是：
do开头的疑问谓语动词是及物动词或者是非及物动词。
be开头是系动词， 或者是被动语态。
例如：
1）用do提问：
Do you like playing football?
Do they come from No 2 Middle School?
Do I need to say something about the issue?
2) be开头的疑问句：
Are you from Beijing?
Is she a student?
I’m not going, am I?
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