85问答库 > 将质粒转化入大肠杆菌怎么保存

将质粒转化入大肠杆菌怎么保存

2025-04-07 11:14:46
推荐回答（1个）
回答1：

如果想保存质粒的话建议保存菌种的同时提取质粒保存于负二十度；保藏已转入质粒的大肠杆菌，短期几个月的话可以直接把LB里生长的菌液保存在4度，或接到LB平板，生长过夜后保存在4度，长期保存的话，菌液加甘油保存在-20度，想放很久不用的话最好加甘油保存在-80或者液氮中。甘油浓度20%就可以了。

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