85问答库 > 求生物高手！转基因食品检测内源基因的外源基因所用的引物序列？是个表格，一定要表格的！！！

求生物高手！转基因食品检测内源基因的外源基因所用的引物序列？是个表格，一定要表格的！！！

2025-03-02 10:25:17

推荐回答（5个）

回答1：

引物名称引物序列
3’AOX 5'-GGC AAA TGG CAT TC TGA CAT-3'
3'AD 5'-AGA TGG TGC ACG ATG CAC AG-3'
3'BD 5'-TAA GAG TCA CTT TAA AAT TTG TAT C-3'
5’AOX1 5'-GAC TGG TTC CAA TTG ACA AGC-3'
A-FACTOR 5'-TAC TAT TGC CAG CAT TGC TGC-3'
BAC1 5'-AAC CAT CTC GCA AAT AAA TA-3'
BAC2 5'-ACG CAC AGA ATC TAG CGC TT-3'
BGH rev 5'-TAG AAG GCA CAG TCG AGG C-3'
CMV-F 5'-CGC AAA TGG GCG GTA GGC GTG-3'
CMV-R 5'-GTT CAC GGT GCC CTC C-3'
DsRed1-C-r 5'-AGC TGG ACA TCA CCT CCC ACA ACG-3'
DsRed1-N-f 5'-GTA CTG GAA CTG GGG GGA CAG-3'
EBV Reverse 5'-TGT GGT TTG TCC AAA CTC AAT C-3'
EBV-R 5'-GTG GTT TGT CCA AAC TCA TC-3'
GAL4 AD-F 5'-TAC CAC TAC AAT GGA TG-3'
GAL4 BD 5'-TCA TCG GAA GAG AGT AG-3'
GLP1 5'-TGT ATC TTA TGG TAC TGT AAC TG-3'
GLP2 5'-CTT TAT GTT TTT GGC GTC TTC CA-3'
M13(-96) 5'-CCC TCA TAG TTA GCG TAA CG-3'
M13F 5'-TGT AAA ACG ACG GCC AGT-3'
M13F（-47） 5'-CGC CAG GGT TTTC CCA GTC ACG AC-3'
M13R 5'-CAG GAA ACA GCT ATG ACC-3'
M13R（-48） 5'-AGC GGA TAA CAA TTT CAC ACA GGA-3'
P24990 5'-TCATCGGAAGCGAGTAG-3'
P31430 5'-CGT TTT AAAACCTAAGAGTCAC-3'
P12584 5'-TTT TCA GTA TCT ACG AT-3’
P17110 5'-TAC CAC TAC AAT GGA TG-3’
PAS2-1F 5'-TCA TCG GAA GCG AGT AG-3'
PAS2-1R 5'-CGT TTT AAA ACC TAA GAG TCA C-3'
PB42ADF 5'-CCA GCC TCT TGC TGA GTG GAG ATG-3'
PB42ADR 5'-AAG CCG ACA ACC TTG ATT GGA G-3'
pbd-gal4-f 5'-GCC TCT AAC ATT GAG ACA GC-3'
pbd-gal4-r 5'-AAG AGT TAC TCA AGA ACA AGA A-3'
pbi-121(35s) 5'-GAC GCA CAA TCC CAC TAT CC-3'
PBV220F 5'-AAG AAG GGC AGC ATT CAA AG-3'
PBV220R 5'-CTG CGT TCT GAT TTA ATC TG-3'
PCDNA3.0-F2 5'-CTC TGG CTA ACT AGA GAA CC-3'
PCDNA3.0-R2 5'-GGC AAC TAG AAG GCA CAG TC-3'
PCDNA3.1F 5'-CTA GAG AAC CCA CTG CTT AC-3’
PCDNA3.1R 5'-TAG AAG GCA CAG TCG AGG-3’
pCEP Frorward 5'-AGC AGA GCT CGT TTA GTG AAC CG-3'
PCEP-F 5'-AGA GCT CGT TTA GTG AAC CG-3'
PCMV-F 5'-TCT AAA AGC TGC GGA ATT GT-3'
PCMV-F 5'-TTC CAA AAT GTC GTA ATA AC-3'
PCMV-R 5'-TCC AAA CTC ATC AAT GTA TC-3'
PCMV-R 5'-ATT ATA GAG GAC ACC TAG TC-3'
PDONR-F 5'-TCG CGT TAA CGC TAG CAT GGA TCT C-3'
PDONR-R 5'-GTA ACA TCA GAG ATT TTG AGA CAC-3’
PEF-F 5'-TCA AGC CTC AGA CAG TGG TTC-3'
PEGFP-C-3’ 5'-TAT GGC TGA TTA TGA TCA GT-3'
PEGFP-C-5’ 5'-CAT GGT CCT GCT GGA GTT CGT G-3'
PEGFP-N-3’ 5'-CGT CGC CGT CCA GCT CGA CCA G-3'
PEGFP-N-5’ 5'-TGG GAG GTC TAT ATA AGC AGA G-3'
pETup 5'-ACG ATG CGT CCG GCG TAG AGG A-3'
PGEX-3’ 5'-CCG GGA GCT GCA TGT GTC AGA GG-3'
PGEX-5’ 5'-GGG CTG GCA AGC CAC GTT TGG TG-3'
PIRES2-EGFP-F 5'-GTA GGC GTG TAC GGT GGG AG-3'
PIRES3-EGFP-R 5'-AAC GCA CAC CGG CCT TAT TC-3'
pMAL-C2X-F 5'-TGC GTA CTG CGG TGA TCA AC-3’
pMAL-C2X-R 5'-CTG CAA GGC GAT TAA GTT GG-3’
PQE30F 5'-TGA GCG GAT AAC AAT TTC AC-3'
PQE30R 5'-GTT CTG AGG TCA TTA CTG G-3'
pTARGET-SEQ 5'-TTA CGC CAA GTT ATT TAG GTG ACA-3’
PTRC99C-F 5'-TTG CGC CGA CAT CAT AAC-3’
PTRC99C-R 5'-CTG CGT TCT GAT TTA ATC TG-3’
PTRCHISA-F 5'-CAT GGT ATG GCT AGC ATG AC-3’
PTRCHISA-R 5'-CAG GCT GAA AAT CTT CTC TC-3’
RVP3 5'-CTA GCA AAA TAG GCT GTC CC-3'
RVP4 5'-GAC GAT AGT CAT GCC CCG CG-3'
S.TAG 5'-CGA ACG CCA GCA CAT GGA CA-3'
S1 5'-CAA CGT GAA AAA ATT ATT ATT CGC-3’
S6 5'-GTA AAT GAA TTT TCT GTA TGA GG-3’
SP6 5'-ATT TAG GTG ACA CTA TAG-3'
T3 5'-ATT AAC CCT CAC TAA AGG GA-3'
T7 5'-TAA TAC GAC TCA CTA TAG GG-3'
T7（17BASE） 5'-ACA TCC ACT TTG CCT TTC TC-3'
T7TER 5'-TGC TAG TTA TTG CTC AGC GG-3'
TRXF 5'-CAT ATG AGC GAT AAA ATT ATT CAC-3'
TRXR 5'-GGA TCC CTT GTC ATC GTC ATC ACC A-3'
malEfor 5'-GGT CGT CAG ACT GTC GAT GAA GCC-3'
pTrcHis-fw 5'-TTA AAG AGG TAT ATA TTA ATG TAT CG-3'
Xpressfor 5'-AGC ATG ACT GGT GGA CAG-3'
pTrcHis-rv 5'-ATC TGT ATC AGG CTG AAA ATC-3'
N-CMV-30 5'-ATA ACC CCG CCC CGT TG-3'
C-CMV-24 5'-TTA GGA CAA GGC TGG TGG-3'
CMV-Profor 5'-ATG GGC GGT AGG CGT G-3'
pCAMBIA1301-5'(ECORI) 5'-CCA GGC TTT ACA CTT TAT GC-3'
pCAMBIA1301-3'(HindIII) 5'-GCG ATT AAG TTG GGT AAC GC-3'
T7 EEV 5'-ATG TCG TAA TAA CCC CGC CCC G-3'
pDBLeu-5' 5'-GAA TAA GTG CGA CAT CAT CAT C- 3’
pDBLeu-3' 5'-CAG ATG GAA CGG TCT TTA AAT G-3’
HGHrev 5'-CTG GGG AGG GGT CAC AGG G-3'
PSG5(BAMHI)-F 5'-GCT GGT TAT TGT GCT GTC TC-3'
PSG5-R(SV40) 5'-AAA CCA CAA CTA GAA TGC AGT-3'
M13R(-26) 5'-CAG GAA ACA GCT ATG AC-3'
pBRforBam 5'-CTT GGA GCC ACT ATC GAC-3'
pBRrevBam 5'-GGT GAT GTC GGC GAT ATA GG-3'
pBRforEco 5'-AAT AGG CGT ATC ACG AGG C-3'
pBRrevHind 5'-GCG TTA GCA ATT TAA CTG TG-3'
c-mycrev 5'-CTC TTC TGA GAT GAG TTT TTG-3'
pGAL4-ADrv 5'-GAA CTT GCG GGG TTT TTC-3'
pGAL4-BDrv 5'-AAT CAT AAG AAA TTC GCC C-3'
pMD19T490R 5'-CACAGGAAACAGCTATGACC-3'
Psos5' 5'-CCA AGA CCA GGT ACC ATG-3'
Psos3' 5'-GCC AGG GTT TTC CCA GT-3'
Primer TriplEx-LD-5´ 5'-CTC GGG AAG CGC GCC ATT GTG TTG G-3'
Primer TriplEx-LDrv-3' 5'-ATA CGA CTC ACT ATA GGG CGA ATT GGC-3'
UB-F 5'-TCA GTG TTA GAC TAG TAA ATT-3'
27F 5'-AGA GTT TGA TCC TGG CTC AG-3’
1492R 5'-GGY TAC CTT GTT ACG ACT T-3’
PTR5 5'-CTC CGA GAT CTG GAC GAG C-3'
pLXSN-F CTT GAA CCT CCT CGT TCG AC
pLXSN-R GTT GCT GAC TAA TTG AGA TG
pLEXA-F CGT CAG CAG AGC TTC ACC AT
pLEXA-R TAA AAC CTA AGA GTC ACT TT
pLNCX-F AGC TCG TTT AGT GAA CCG TCA GAT CG
pLNCX-R ACC TAC AGG TGG GGT CTT TCA TTC CC
pDSRED-N-R TGA AGC GCA TGA ACT CCT TG
mtDNA-D-LoopF AGG ACT Acg gct tga aaa gc
mtDNA-D-LoopR atg tgc ctg acc gag gaa cca g
H1primer CGCTATGTGTTCTGGGAAAT
pRECEIVER-M03F 5'-GCGGTAGGCGTGTACGGT-3'
pRECEIVER-M03R 5'-CCGGACACGCTGAACTTGT-3'
ACYCDuetUP1Primer #71178-3 GGATCTCGACGCTCTCCCT
DuetUP2 Primer TTGTACACGGCCGCATAATC
DuetDOWN1 GATTATGCGGCCGTGTACAA
1525R 5'-AgAAAggAggTgATCcAgcc-3'
pBAD_F ATGCCATAGCATTTTTATCC
pBAD_R GATTTAATCTGTATCAGG
AUG1F 5'-CAATTTACATCTTTATTTATTAACG-3'
AUG1R 5'-GAAGAGAAAAACATTAGTTGGC-3'
V5rev 5'-ATC GAG ACC GAG GAG AGG -3'
EGFP-Nrev 5'-CGT CGC CGT CCA GCT C-3'
TKPAreverse 5΄-CTTCCGTGTTTCAGTTAGC-3΄
pDsRED-express-C1-F TCC CAC AAC GAG GAC TAC AC
DEST22F 5’-tataacgcgtttggaatcact-3’
DEST22R 5’-agccgacaaccttgattggagac-3’
pBMN_5' gcttggatacacgccgc
pYes2.R TCG GTT AGA GCG GAT GTG
pSG5-3 GAAATTTGTGATGCTATTGC
pMSCV-5' CCCTTGAACCTCCTCGTTCGACC
pMSCV-3' GAGACGTGCTACTTCCATTTGTC

内参通用引物 16SDNAs
primer sequence
27F 5’-AGAGTTTGATCCTGGCTCAG-3’
515F 5’-GTGCCAGCMGCCGCGGTAA-3’
21F 5’-TTCCGGTTGATCCTGCCGGA-3’
312F 5’-TCCTACGGGAGGCAGCAGCT-3
1492R 5’-GGYTACCTTGTTACGACTT-3’
1525R 5'-AgAAAggAggTgATCcAgcc-3'
ITS1 TCCGTAGGTGAACCTGCGG
ITS4 TCCTCCGCTTATTGATATGC

真菌通用引物？
nu-SSU-0817-5’ TTAGCATGGAATAATRRAATAGGA
nu-SSU-1536-3’ ATTGCAATGCYCTATCCCCA

这是我在百度文库里找到的，说是质粒的常用引物序列。不知对你的朋友有没有帮助。