你们黄曲霉毒素B1是怎么检测的?用什么方法学检测的?

2025-04-07 13:00:10
推荐回答(1个)
回答1:

通用方法

薄膜层析法和液相色谱法是目前国内绝大多数检测机构都在使用的方法。由于其检测周期长,程序复杂所需试剂繁多等缺点已远远不能满足现代检测要求.随着现代科学技术的不断发展,特别是免疫学生物化学,分子生物学的不断发展人们已创建了不少快速,简便特异,敏感低耗且适用的黄曲霉毒素检测方法.而且以金标试纸为代表的这些方法已经被先进国家所广泛使用,引进和消化这些先进的方法是我们检测领域的当务之急.免疫亲和柱法优点很多但由于检测费用过高,而无法普及.而一步式黄曲霉毒素检测金标试纸法似乎更适用于中国值得推广。薄层层析(Thin-Layer Chromatography,TLC)是在黄曲霉毒素研究方面应用最广的分离技术.自1990年它被列为AOAC (Association of Official Agricultural Chemists)标准方法,该方法同时具有定性和定量分析黄曲霉毒素的功能.2、液相色谱法液相色谱(Liquid Chromatography,LC)与薄层层析在许多方面具有相似性二者互相补充.通常用TLC进行前期的条件设定,选择适宜的分离条件后再用LC进行黄曲霉毒素的定量测定。3、免疫化学分析方法利用具有高度专一性的单克隆抗体或多克隆抗体设计的黄曲霉毒素的免疫分析方法也是最常用的黄曲霉毒素检测方法.这类方法通常包括放射免疫分析方法(Radioimmunoassay,RIA),酶联免疫法(Enzyme-linked of Immunosorbent Assay,ELISA)和免疫层析法(Immunoaflinity Column Assay,ICA).它们均可以对黄曲霉毒素进行定量测定。

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